APA
Click to copy
Golden, G. J., Wu, V., Hamilton, J., Amses, K. R., Shapiro, M. R., Japp, A. S., … Betts, M. R. (2024). Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset. BioRxiv.
Chicago/Turabian
Click to copy
Golden, Gregory J., V. Wu, J. Hamilton, Kevin R. Amses, Melanie R. Shapiro, A. S. Japp, Chengyang Liu, et al. “Immune Perturbations in Human Pancreas Lymphatic Tissues Prior to and after Type 1 Diabetes Onset.” bioRxiv (2024).
MLA
Click to copy
Golden, Gregory J., et al. “Immune Perturbations in Human Pancreas Lymphatic Tissues Prior to and after Type 1 Diabetes Onset.” BioRxiv, 2024.
BibTeX Click to copy
@article{gregory2024a,
title = {Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset},
year = {2024},
journal = {bioRxiv},
author = {Golden, Gregory J. and Wu, V. and Hamilton, J. and Amses, Kevin R. and Shapiro, Melanie R. and Japp, A. S. and Liu, Chengyang and Pampena, M. and Kuri-Cervantes, L. and Knox, James J. and Gardner, Jay S. and Atkinson, M. and Brusko, T. and Prak, Eline T. Luning and Kaestner, Klaus H. and Naji, Ali and Betts, Michael R.}
}
Autoimmune destruction of pancreatic β cells results in type 1 diabetes (T1D), with pancreatic immune infiltrate representing a key feature in this process. Studies of human T1D immunobiology have predominantly focused on circulating immune cells in the blood, while mouse models suggest diabetogenic lymphocytes primarily reside in pancreas-draining lymph nodes (pLN). A comprehensive study of immune cells in human T1D was conducted using pancreas draining lymphatic tissues, including pLN and mesenteric lymph nodes, and the spleen from non-diabetic control, β cell autoantibody positive non-diabetic (AAb+), and T1D organ donors using complementary approaches of high parameter flow cytometry and CITEseq. Immune perturbations suggestive of a proinflammatory environment were specific for T1D pLN and AAb+ pLN. In addition, certain immune populations correlated with high T1D genetic risk independent of disease state. These datasets form an extensive resource for profiling human lymphatic tissue immune cells in the context of autoimmunity and T1D.